BRCA1 anticorps (AA 762-1315)

N° du produit ABIN7468062

Cet anticorps Souris Monoclonal détecte spécifiquement BRCA1 dans WB, IHC, ELISA, IF, IHC (p), IP, ICC et ChIP. Il présente une réactivité envers Humain.

Aperçu rapide pour BRCA1 anticorps (AA 762-1315) (ABIN7468062)

Antigène: BRCA1 (Breast Cancer 1 (BRCA1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BRCA1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Classe de qualité: KO Validated

Clone: 17F8

Détail du produit anti-BRCA1 anticorps

Épitope: AA 762-1315

Specificité: This antibody does not recognize the delta exon 11 splice variant of BRCA1. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm.

Réactivité croisée: Humain, Souris

Purification: Protein G purified

Immunogène: Protein fragment expressed in E. coli corresponding to amino acids 762-1315.

Isotype: IgG1

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1 μg/mL. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: 293T , A431 , HeLa Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.55 mg/mL

Buffer: PBS, No Preservative

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur BRCA1

Antigène: BRCA1 (Breast Cancer 1 (BRCA1))

Autre désignation: BRCA1 DNA repair associated

Sujet:

Synonyms: BRCA1 DNA repair associated , BRCAI , BRCC1 , BROVCA1 , FANCS , IRIS , PNCA4 , PPP1R53 , PSCP , RNF53

Background: This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009]

Poids moléculaire: 208 kDa

ID gène: 672

UniProt: P38398

Pathways: Cycle Cellulaire, Réparation de l'ADN, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus